溶血
化学
表儿茶素没食子酸盐
多酚
没食子酸表没食子酸酯
食品科学
红细胞
生物化学
抗氧化剂
溶解
激进的
生物
免疫学
作者
Anqi Zhang,Qin Zhu,Yan Shun Luk,Ka Yan Ho,Kwok‐Pui Fung,Zhenyu Chen
出处
期刊:Life Sciences
[Elsevier]
日期:1997-06-01
卷期号:61 (4): 383-394
被引量:101
标识
DOI:10.1016/s0024-3205(97)00395-0
摘要
Jasmine green tea is an excellent source of natural polyphenol antioxidants including mainly (-) epicatechin (EC), (-) epicatechin gallate (ECG), (-) epigallocatechin (EGC) and (-) epigallocatechin gallate (EGCG). The present study was to test our hypothesis that ingestion of jasmine tea would protect red blood cell (RBC) membrane from free radical-induced oxidation if jasmine tea epicatechin isomers could be absorbed and circulated in blood. When incubated with RBC suspension, all four epicatechin isomers purified from jasmine tea exhibited a strong protection for RBC membrane to hemolysis induced by 2,2′-azo-bis(2-amidinopropane) dihydrochloride (AAPH), an azo free radical initiator. The inhibitory effect was dose-dependent at the concentrations of 2.5 μiM to 40 μM. The fatty acid analysis revealed that all four epicatechin isomers significantly prevented loss of arachidonic acid (20:4n-6) and docosahexaenoic acid (22:6n-3) in RBC incubated under the same conditions. Although the in vitro antioxidative activity of EGCG and ECG was more effective than EGC and EC, the latter two isomers were more important in vivo in scavenging free radicals. This was because only EGC and EC instead of EGCG and ECG were circulating in blood stream after a gavage-dose of 100 mg jasmine tea GTP mixture. In fact, ingestion of jasmine tea GTP extracts was associated with a significant decrease in susceptibility of RBC to hemolysis in rats.
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