富血小板血浆
伤口愈合
材料科学
傅里叶变换红外光谱
生理盐水
扫描电子显微镜
表皮(动物学)
化学
生物医学工程
基质(化学分析)
医学
血小板
外科
内科学
解剖
色谱法
复合材料
物理
量子力学
作者
Xiaoxuan Lei,Y. Yang,Guiqiu Shan,Yingen Pan,Biao Cheng
标识
DOI:10.1088/1748-605x/ab0060
摘要
Acellular dermal matrix (ADM) and platelet-rich plasma (PRP) have each been used in wound healing. There are a few reports on the application of ADM with PRP in skin full-thickness defect models. In this study, the microstructure of ADM/PRP freeze-dried dressing was observed by scanning electron microscope. The PRP, ADM and PRP/ADM samples were recorded by Fourier transform infrared (FTIR) spectrometer by the KBr methods. The concentration of growth factor was measured by enzyme-linked immunosorbent assay. Then, we made a mice full-thickness defect model and treated it with saline, PRP, ADM and PRP/ADM, respectively. The wound size was measured and calculated on days 3, 5, 7, 10 and 14. The obtained results demonstrated that the ADM/PRP freeze-dried dressing resulted in earlier collagen development. FTIR analysis confirmed the integration of ADM and PRP in the prepared PRP/ADM scaffold. The concentration of bFGF, EGF, TGF-β1 and PDGF-BB in the ADM/PRP group was lower than that in the PRP group. The wound healing rate of the ADM/PRP group was significantly promoted. Furthermore, the ADM/PRP group had significant revascularization, rapid epithelialization and a well-differentiated epidermis. The collagen fibers were regularly arranged. Accordingly, ADM/PRP freeze-dried dressing promotes wound healing and can be used in the skin full-thickness wound.
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