Optimization of extracting technology of collagen of antler base of cervus nippon by response surface methodology

Objective With the antler base of Cervus Nippon,extracting technology of collagen was optimized by the response surface methodology.Methods Based on single factor experiments,a four-factor and four-level Box-Behnken design was carried out to investigate the effects of pH value,enzyme loading,hydrolysis temperature,hydrolysis time with collagen extraction ratio as index.Effects of the 4 factors on collagen extraction ratio were analyzed by the method of response surface.Results According to the actual situation,the optimal extracting technology of collagen of antler base of Cervus Nippon was revised as follows:under the condition of pH1.8,enzyme loading 4%,hydrolysis temperature 37 ℃ and hydrolysis time 4 h,collagen extraction ratio of antler base of Cervus Nippon could reach 80.10%.Conclusion It was influenced by many elements for the extraction of collagen of antler base of Cervus Nippon.Therefore,analyzing the effects of single factors and interaction of each other and optimizing experimental methods and conditions would greatly improve the extraction ratio of collagen of antler base of Cervus Nippon.