Five heavy metals in soils cultivating Dictyophora rubrovalvata M.Zang.Ji.et Liu.from Northwestern Guizhou province were investigated and the pollution assessment was made by evaluation method of single pollution index and integrated pollution index(Nemero index).The results showed that the soils No.1 and No.2 was polluted less at pollution level Ⅱ.The other regions belonged to moderate or heavy pollution level.Cd and Cu were the main elements of heavy metal pollution as the single-factor pollution index was high.The correlation between the four elements(Cd,Cu,Hg,As) was very significant,indicating that these four elements might have the same source of heavy metals contamination or combined pollution.Principal component analysis showed that the main heavy metal pollution might come from outside factors.