Comparative Study on Clematis Chinesis and Clematis uncinata by HPLC in vitro and in vivo

Objective To compare Clematis chinesis of common traditional Chinese medicine and Clematis uncinata of local variety by HPLC fingerprint in vitro and in vivo.MethodsThe fingerprints of Clematis chinesis,Clematis uncinata,medicated plasma of Clematis chinesis,medicated plasma of Clematis uncinata and plasma of blank were determined by HPLC.The common peaks between Clematis chinesis and Clematis uncinata,medicated plasma of Clematis chinesis,medicated plasma of Clematis uncinata were analyzed.ResultsThere were 10 common peaks in Chromatographic fingerprint of Clematis chinesis and Clematis uncinata,there were 5 common peaks in Chromatographic fingerprint of medicated plasma of Clematis chinesis and medicated plasma of Clematis uncinata.ConclusionFurther studies on common peaks of Clematis chinesis and Clematis uncinata is helpful to elucidate the same generic plants which might have the same effective substance.