甲基化
生物
DNA甲基化
胎儿
CpG站点
表观遗传学
胎盘
基因
亚硫酸氢盐测序
克隆(编程)
分子生物学
差异甲基化区
基因组印记
基因表达
亚硫酸氢盐
男科
遗传学
怀孕
医学
程序设计语言
计算机科学
作者
Changlei Li,Congying Zheng,Jun Liu,Jie Lan,Wenzhe Li,Yong Zhang
出处
期刊:Chinese Journal of Biotechnology
日期:2010-05-01
卷期号:26 (5): 582-587
被引量:1
摘要
The aberrant epigenetic reprogramme is an important cause for abnormal development of nuclear transfer embryos. The objective of this study was to investigate the CpG island methylation profiles and relative expression levels of H19 gene in different tissues of cloned goat fetus. We detected liver, placenta, kidney, lung and heart in the dead cloned goat fetus and the age-matched normal goat fetus (control) by using bisulfite sequencing and real time PCR. Results indicated that methylation levels of the fifth CpG island of H19 gene in dead cloned goat fetus was significant high compared with that in the control in placenta (70% vs 49.41%, P 0.05). Results showed the abnormal DNA methylation proflies of H19 gene occurred in some tissues of cloned goat fetus, which affected normal expression levels of H19 gene, indicating that aberrant DNA methylation reprogramme may be one of the important factors for the death of cloned animals.
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