Hypoglycemic Effect of Humifuse Euphorbia Herb Extract in Diabetic Mice

Objective To explore the hypoglycemic effect of humifuse euphorbia herb extract in diabetic mice and its mechanism of action.Methods Sixty mice were given intravenous injection of 0.25%alloxan solution(80mg·kg-1)though tail vein,and then were intragastricly administrated 50%glucose solution(0.2mL·10g-1)2hours after intravenous injection.After 3days,orbital blood was taken to detect the levels of blood glucose.Mice with blood glucose levels above11.1mmol·L-1 were regarded as the diabetic mice.Fifty diabetic mice were randomly divided into 5groups:normal saline group(negative control group),low-dose humifuse euphorbia herb group(59mg·kg-1·d-1),medium-dose humifuse euphorbia herb group(118mg·kg-1·d-1),high-dose humifuse euphorbia herb group(236mg·kg-1·d-1)and metformin group(positive control group),with 10mice in each group.After successive administration for 14days,levels of blood glucose and serum insulin were measured and pathological changes in pancreatic islet were observed.Results 1)Blood glucose:Compared with both negative control group and low,mediun-dose humifuse euphorbia herb group and before medication,blood glucose levels significantly decreased in both high-dose humifuse euphorbia herb group and metformin group(P0.05).However,no obvious decrease in blood glucose levels were found after treatment in negative control group,low-dose humifuse euphorbia herb group and medium-dose humifuse euphorbia herb group(all P0.05).2)insulin:Compared with negative control group,low-dose humifuse euphorbia herb group or medium-dose humifuse euphorbia herb group,serum insulin levels markedly increased in both high-dose humifuse euphorbia herb group and metformin group(all P0.05).No significant differences in serum insulin levels were found among negative control group,low-dose humifuse euphorbia herb group and medium-dose humifuse euphorbia herb group(all P0.05).3)Pathological change of the pancreas:Pathological analysis showed obvious decrease in the number the pancreatic islets with damaged structures,blurred boundaries,disorderedβcell arrangement and partial degeneration or necrosis in negative control group,but significant increase in the number the pancreatic islets with complete structures,clear boundaries,orderlyβcell arrangement,normal cell morphology,abundant cytoplasm,clear nucleus and mild degeneration or necrosis in humifuse euphorbia herb groups.In addition,metformin treatment improved the morphology of pancreatic islets compared with negative control group.Conclusion Humifuse euphorbia herb extract exerts has hypoglycemic effect in diabetic mice through protecting pancreatic isletβcells,reducing damage and promoting insulin secretion.