转化(遗传学)
电穿孔
PEG比率
聚乙二醇
农杆菌
生物
微生物学
基因
计算生物学
遗传学
生物化学
财务
经济
作者
Zhaohui Liu,Timothy L. Friesen
出处
期刊:Methods in molecular biology
日期:2011-11-23
卷期号:: 365-375
被引量:73
标识
DOI:10.1007/978-1-61779-501-5_21
摘要
Genetic transformation is an essential tool for the modern study of gene function and the genetic improvement of an organism. The genetic transformation of many fungal species is well established and can be carried out by utilizing different transformation methods including electroporation, Agrobacterium, biolistics, or polyethylene glycol (PEG)-mediated transformation. Due to its technical simplicity and common equipment requirements, PEG-mediated transformation is still the most commonly used method for genetic transformation in filamentous fungi. Here, we describe a PEG-based protocol developed for genetic transformation of Stagonospora nodorum, a fungal pathogen of wheat. This protocol is directly applicable to other fungi especially those in the Dothideomycete class of fungi.
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