Simultaneous Determination of 2″-O-rhamnosyl Vitexin and Vitexin in Chinese Hawthorn Leaf and Its Extract by RP-HPLC

Aim To establish an RP-HPLC method for simultaneous determination of 2″-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract. Methods Chromatography was carried out on Kromasil C_ 18 column (250 mm × 4.6 mm, 5 μm), using THF-CH_3CN-H_2O-H_3PO_4 (30∶5∶125∶ 0.1 ) as the mobile phase at a flow rate of 1.0 mL·min -1 . The UV detection wavelength was 270 nm. Results The linear range of 2″-O-rhamnosyl vitexin and vitexin were 0.106 4 μg- 2.1280 μg ( r =0.999 1 ) and 0.139 2 μg-2.784 0 μg ( r =0.999 3 ), respectively. The average recoveries of 2″-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf were 99.2% (RSD = 2.80% , n= 6) and 100.6% (RSD = 2.84%, n = 6), respectively. Conclusion This method is reproducible, simple, precise, and rapid for simultaneous determination of 2″-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract, thereby providinge the basis for quality specification of Chinese hawthorn leaf and its extract.