THE STUDY OF RELATIONSHIP BETWEEN RADIATION-INDUCED APOPTOSIS OF HUMAN FIBROBLAST AND ITS RADIOSENSITIVITY

To investigate the radiation-induced apoptosis of human fibroblasts with different radiosensitivity,the methods of TUNEL and FCM were employed to test the apoptosis of cells treated with 60 Co γ-rays. The results of experiments indicated that:(1) Surviving fraction experiment identified the No 6 fibroblast cells have a high radiosensitivity than that of No 8 cells after exposured to 60 Co γ-rays; (2) Apoptosis rate has a positive relationship with radiosensitivity by the methods of TUNEL and FCM, in accordance with the result made by the surviving fraction experiment; (3) Both apoptosis rate and radiosensitivity increased within the 48 hours after radiation.In this conclusion is that: The changing of apoptosis rate can reflect the radiosensitivity level of cell line; the changing of cellular apoptosis rate and radiosensitivity were time-dependant after radiation. it is possible that apoptosis may be an important marker for judging cellular radiosensitivity.