Acyl migration during methanolysis of palm oil and methanol catalyzed by Lipozyme RM IM

Acyl migration during methanolysis of palm oil and methanol catalyzed by 1,3-positional specific lipase(Lipozyme RM IM) in solvent free system was studied,including acyl migration of 1,2-diglyceride to 1,3-diglyceride and 2-monoglyceride to 1(3)-monoglyceride.The effects of substrate molar ratio,reaction temperature and enzyme dosage on the acyl migration were discussed.The results showed that when molar ratio of methanol to oil was lower than 3:1,acyl migration increased with reaction time increasing;when the reaction temperature was in the range of 50-60 ℃,acyl migration increased with the increase of reaction temperature and reaction time.When enzyme dosage increased from 4% to 8%,acyl migration increased with reaction time increasing.