石油醚
乙酸乙酯
化学
抗菌剂
氯仿
乙醇
变形链球菌
抗菌活性
最小抑制浓度
肉汤微量稀释
色谱法
细菌
食品科学
有机化学
生物
萃取(化学)
遗传学
作者
Jin Xiao,Yuan Liu,Yu Ling Zuo,Ji Yao Li,Ling Ye,Xue Zhou
标识
DOI:10.1016/j.archoralbio.2006.03.014
摘要
Nidus Vespae is the honeycomb of Polistes Olivaceous (De Geer), P. Japonicus Saussure, and Parapolybiavaria Fabricius. It is known to have a number of pharmacological effects, including antimicrobial, anti-inflammatory, anti-virus, anti-tumor and anesthetic properties. The present study evaluated the antimicrobial activity and acid inhibition properties of extracts and chemical fractions of Nidus Vespae. The raw material was first extracted using 95% ethanol/water. Subsequent fractions were prepared from this extract using cyclohexane/ethyl acetate, petroleum ether/ethyl acetate, and chloroform/methanol. For the antimicrobial activity assays, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined using the microdilution method. The chloroform/methanol (Chl/MeOH) fraction showed the highest antibacterial activities with a MIC of 8-16mg/ml and an MBC of 16-32mg/ml. In addition, the extract and chemical fractions of Nidus Vespae showed a remarkable capacity for inhibiting the acid production of common oral bacteria at sub-MIC concentrations. Sub-MIC levels of the petroleum ether/ethyl acetate fraction significantly inhibited acid production by Streptococcus mutans ATCC 25175. The significant antiacidogenic activity demonstrated by Nidus Vespae shows it to be a promising source of novel anticariogenic agents.
科研通智能强力驱动
Strongly Powered by AbleSci AI