单克隆抗体
分子生物学
抗体
基因
乙酰化
生物
遗传学
病毒学
计算生物学
作者
Evelyne Cerato,Stéphane Birklé,Jacques Portoukalian,A. Mezazigh,Jean‐François Chatal,Jacques Aubry
出处
期刊:Hybridoma
[Mary Ann Liebert]
日期:1997-08-01
卷期号:16 (4): 307-316
被引量:27
标识
DOI:10.1089/hyb.1997.16.307
摘要
Despite the weak immunogenicity of gangliosides, a limited number of highly specific murine monoclonal antibodies (MAbs) were elicited. This study investigated the reactivity and the structure of the VH and V kappa genes of nine hybridomas obtained from independent fusions producing antibodies against disialogangliosides GD2 and GD3 and their O-acetylated derivatives. These antibodies depended on four types of V kappa genes. They were also encoded by VH genes of the J558 family (5 out of 9) and occasionally by VH genes of the S107, 7183, and 3609 families, rearranged with a variety of DH and JH genes. The 8B6 and 7H2 MAbs specific for GD2-O-acetylated, respectively, used the VH gene of the S107 and 7183 families. The length of H chain CDR3 ranged from 8 to 11 amino acids. A set of S107 and 3609 germline genes closed from A/J murine fetal liver and matched with the VH segment of hybridomas 8B6 and 10B8 revealed somatic mutations. Although the relative number of sequences does not preclude any formal conclusions regarding the preferential use of V genes in the immune recognition of carbohydrate structures, our results clearly indicate that MAbs directed to very similar structures as GD2 and GD3 were encoded by different VH and V kappa genes.
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