太空飞行
甲基化
生物
仆从
短小芽孢杆菌
基因组
DNA甲基化
国际空间站
遗传学
作者
Samantha M. Waters,S. Marshall Ledford,Amanda L. Wacker,Sonali Verma,Bianca M. Serda,Jordan M. McKaig,Joseph Varelas,Patrick M. Nicoll,Kasthuri Venkateswaran,David J. Smith
标识
DOI:10.1017/s1473550421000343
摘要
Abstract Bacillus pumilus SAFR-032, an endospore-forming bacterial strain, was investigated to determine its methylation pattern (methylome) change, compared to ground control, after direct exposure to space conditions onboard the International Space Station (ISS) for 1.5 years. The resulting ISS-flown and non-flown strains were sequenced using the Nanopore MinION and an in-house method and pipeline to identify methylated positions in the genome. Our analysis indicated genomic variants and m6A methylation increased in the ISS-flown SAFR-032. To complement the broader omics investigation and explore phenotypic changes, ISS-flown and non-flown strains were compared in a series of laboratory-based chamber experiments using an X-ray irradiation source (doses applied at 250, 500, 750, 1000 and 1250 Gy); results show a potentially higher survival fraction of ISS-flown DS2 at the two highest exposures. Taken together, results from this study document lasting changes to the genome by methylation, potentially triggered by conditions in spaceflight, with functional consequences for the resistance of bacteria to stressors expected on long-duration missions beyond low Earth orbit.
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