脂肪组织
免疫标记
交感神经系统
PRDM16
脂肪细胞
白色脂肪组织
内分泌学
生物
内科学
生物发生
交感神经支配
表型
细胞生物学
神经科学
医学
免疫组织化学
基因
血压
生物化学
作者
Jingyi Chi,Zhuhao Wu,Chan Hee J. Choi,Lily Nguyen,Saba Tegegne,Sarah E. Ackerman,Audrey Crane,François Marchildon,Marc Tessier‐Lavigne,Paul Cohen
出处
期刊:Cell Metabolism
[Elsevier]
日期:2018-01-01
卷期号:27 (1): 226-236.e3
被引量:191
标识
DOI:10.1016/j.cmet.2017.12.011
摘要
While the cell-intrinsic pathways governing beige adipocyte development and phenotype have been increasingly delineated, comparatively little is known about how beige adipocytes interact with other cell types in fat. Here, we introduce a whole-tissue clearing method for adipose that permits immunolabeling and three-dimensional profiling of structures including thermogenic adipocytes and sympathetic innervation. We found that tissue architecture and sympathetic innervation differ significantly between subcutaneous and visceral depots. Subcutaneous fat demonstrates prominent regional variation in beige fat biogenesis with localization of UCP1+ beige adipocytes to areas with dense sympathetic neurites. We present evidence that the density of sympathetic projections is dependent on PRDM16 in adipocytes, providing another potential mechanism underlying the metabolic benefits mediated by PRDM16. This powerful imaging tool highlights the interaction of tissue components during beige fat biogenesis and reveals a previously undescribed mode of regulation of the sympathetic nervous system by adipocytes.
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