癌症研究
基因敲除
下调和上调
细胞周期
基因沉默
细胞培养
胶质瘤
小发夹RNA
细胞
分子生物学
长非编码RNA
细胞凋亡
癌细胞
小干扰RNA
小RNA
作者
Fengquan Gao,Yan Du,Yun Zhang,Deshuai Ren,Jiazhi Xu,Dagang Chen
出处
期刊:Gene
[Elsevier]
日期:2020-02-05
卷期号:726: 144196-144196
被引量:7
标识
DOI:10.1016/j.gene.2019.144196
摘要
Accumulating evidence has indicated the important roles of circular RNAs (circRNAs) in different tumors. However, their detailed regulatory mechanisms in glioma are not fully understood. In this study, the functional role of a novel circRNA, circ-EZH2, was investigated by cell counting kit-8 (CCK-8), colony formation, flow cytometry, and transwell experiments. The regulatory mechanism of circ-EZH2 was explored by bioinformatics analysis, quantitative real-time PCR (qRT-PCR), Western blot and dual-luciferase reporter assay. We identified that circ-EZH2 was overexpressed in glioma tissues and cell lines. Further studies revealed that ectopic expression of circ-EZH2 significantly promoted cell growth, migration and invasion but inhibited cell apoptosis. By contrast, silencing of circ-EZH2 induced the opposite effects. Additionally, we found circ-EZH2 served as a miRNA sponge for miR-1265 to release its suppression on DDAH1 and CBX3. Rescue assays further revealed that the oncogenic function of circ-EZH2 was partly dependent on its modulation of DDAH1 and CBX3. Our study unraveled a novel molecular pathway in glioma and may provide a new perspective for the treatment of glioma.
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