分析物
光刻胶
微流控
免疫分析
聚苯乙烯
色谱法
纳米技术
材料科学
光刻
聚合物
化学
抗体
图层(电子)
免疫学
复合材料
生物
作者
Edward Eteshola,Deborah Leckband
标识
DOI:10.1016/s0925-4005(00)00640-7
摘要
This report describes the first preliminary evaluation of a heterogeneous sandwich enzyme-linked immunoassay in a PDMS microfluidic device. The PDMS devices were fabricated using replica molding against a patterned photoresist generated by photolithographic techniques. With this experimental setup, the microfluidic sensor chip was successfully used to quantify a model analyte (sheep IgM) with sensitivity down to 17 nM. The conventional blocking cocktail used in nearly all polystyrene microtiter plate-based ELISA assays failed to block the nonspecific adsorption of the analyte and secondary antibody. This report describes the successful use of surface modification chemistries and a modified blocking cocktail to reduce background due to nonspecific adsorption and to thereby increase the sensitivity of the assay. These protocols can be extended to the detection of a variety of analytes by immunoassay in PDMS microchannels.
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