毕赤酵母
水解
酶
糖基化
生物化学
化学
基质(水族馆)
水解酶
蛋白质工程
N-连接糖基化
毕赤酵母
大肠杆菌
重组DNA
生物
聚糖
糖蛋白
生态学
基因
作者
Xian Li,Beilei Shi,Jun Huang,Ziyin Zeng,Yu Yang,Lilan Zhang,Jian Min,Chun‐Chi Chen,Rey‐Ting Guo
标识
DOI:10.1186/s40643-023-00648-1
摘要
Abstract Using enzymes to hydrolyze and recycle poly(ethylene terephthalate) (PET) is an attractive eco-friendly approach to manage the ever-increasing PET wastes, while one major challenge to realize the commercial application of enzyme-based PET degradation is to establish large-scale production methods to produce PET hydrolytic enzyme. To achieve this goal, we exploited the industrial strain Pichia pastoris to express a PET hydrolytic enzyme from Caldimonas taiwanensis termed Ct PL-DM. In contrast to the protein expressed in Escherichia coli , Ct PL-DM expressed in P. pastoris is inactive in PET degradation. Structural analysis indicates that a putative N -glycosylation site N181 could restrain the conformational change of a substrate-binding Trp and hamper the enzyme action. We thus constructed N181A to remove the N -glycosylation and found that the PET hydrolytic activity of this variant was restored. The performance of N181A was further enhanced via molecular engineering. These results are of valuable in terms of PET hydrolytic enzyme production in industrial strains in the future. Graphical Abstract
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