Clustering ensemble in scRNA-seq data analysis: Methods, applications and challenges

With the rapid development of single-cell RNA-sequencing techniques, various computational methods and tools were proposed to analyze these high-throughput data, which led to an accelerated reveal of potential biological information. As one of the core steps of single-cell transcriptome data analysis, clustering plays a crucial role in identifying cell types and interpreting cellular heterogeneity. However, the results generated by different clustering methods showed distinguishing, and those unstable partitions can affect the accuracy of the analysis to a certain extent. To overcome this challenge and obtain more accurate results, currently clustering ensemble is frequently applied to cluster analysis of single-cell transcriptome datasets, and the results generated by all clustering ensembles are nearly more reliable than those from most of the single clustering partitions. In this review, we summarize applications and challenges of the clustering ensemble method in single-cell transcriptome data analysis, and provide constructive thoughts and references for researchers in this field.