阿拉伯树胶
乳状液
大豆蛋白
阿拉伯语
化学
糖化血红蛋白
复合材料
材料科学
化学工程
食品科学
生物化学
医学
2型糖尿病
内分泌学
工程类
糖尿病
语言学
哲学
作者
Sirui Feng,Yunsi Guo,Feng Liu,Zhangpeng Li,Kexian Chen,Akihiro Handa,Yue Zhang
标识
DOI:10.1016/j.foodhyd.2022.108168
摘要
Complexation and glycation conjugation are the two most common routes to prepare protein-polysaccharide based encapsulant materials for food and pharmaceutical applications. It is very necessary to investigate the impacts of the two preparation routes on the structures, physicochemical and functional properties of protein-polysaccharide composites at both molecular and macroscopic levels. In this study, soy protein isolate (SPI)-gum Arabic (GA) composites were prepared through complexation and glycated conjugation and characterized by various analytical techniques. The functionality of SPI/GA composites as encapsulants was evaluated by using eugenol emulsion as a model. The composites prepared by conjugation showed better emulsifying and stabilizing abilities, lower release rate but higher skin penetration ability than complexes, which could be attributed to the slightly better interfacial tension reduction ability and electronegativity. The complexation and conjugation abilities of GA with glycinin (11S) and β-conglycinin (7S), two major individual proteins in SPI, were also evaluated by intrinsic fluorescence quenching method, grafting degree determination and molecular docking. The results indicated that 7S showed higher tendency to form conjugates with GA than 11 S while the two proteins had similar complexation affinities. Findings from this work is promising for the development of SPI-polysaccharide based encapsulation system for food and pharmaceutical applications. • SPI-GA composites were prepared by complexation and wet Maillard reaction based-conjugation. • Conjugation could better improve the encapsulation efficiency and storage stability of SPI-GA. • The better functionality of conjugates was contributed to the interfacial tension reduction ability and electronegativity. • The complexation and conjugation abilities of 11S and 7S with GA were firstly evaluated. • GA showed higher tendency to form conjugates with 7S rather than 11S.
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