Effect of Wedelolactone on IL-6、TNF-α and NF - κB p65 Expression in Lipopolysaccharide Induced RAW264.7 Cells

Objective To study the effect of Wedelolactone on IL-6、TNF-α and NF-κB p65 expression in lipopolysaccharide(LPS) induced RAW264.7 cells.Methods The contents of IL-6 and TNF-α were detected by ELISA methods.The expression of NF-κB p65 protein in RAW264.7 cells was measured by Western blot,in the presence or absence of 10 μg.ml-1 lipopolysaccharide(LPS) or Wedelolactone(0.2,2,20μmol.L-1).Results Compared with control group,the contents of IL-6 and TNF-α were significantly induced by LPS,Wedelolactone inhibited the expression of IL-6 and TNF-α significantly elicited by LPS in RAW264.7 cells,in dose dependent manners.LPS-induced the expression of NF-κB p65 protein was significantly inhibited by Wedelolactone in RAW264.7 cells.Conclusion Wedelolactone has anti-inflammatory effect,which might be mediated by down-regulating inflammatory factors IL-6,TNF-α through NF-κB signal pathway.