Isolation of Xylanase Producing Strain and Immobilization of Xylanase

6 xylanase producing strains were screened from the intestinal tract of grass carp,among which the strain with the highest xylanase yield was adopted to detect the enzyme activity of xylanase.Furthermore,the xylanase was immobilized by chitosan and sodium alginate.The results showed that compared with free enzyme,the optimal pH for chitosan immobilized xylanase was higher while for sodium alginate immobilized xylanase was lower.The optimal temperature for the immobilized xylanase was higher than that of free enzyme.The immobilized xylanase also showed broader temperature adaptability.