Preparation and Bio- activive Assay of CD_ 3AK Cell from Umbilical Blood

Objective Objective To study the anti tumor effect of CD 3AK cell prepared from umbilical blood, to explore the recent curative effect on tumor cases and seek better immune index for bio therapy.Methods IL 2 and IL 2+CD 3Ab was used to induce LAK cell and CD 3AK cell from umbilical blood mononuclear cells (UBMC). The expanding number and bio activity of LAK cell and CD 3AK cell were examined in different time after culture, the NK activity of peripheral blood mononuclear cells (PBMC) of 12 cases of malignant tumor patients were examined before and after CD 3AK cell adopting immune therapy as well.Results The number and bio activity(NK killing cancer K562)of LAK and CD 3AK cells reached their peaks on 11th day. The number of LAK and CD 3AK cells were 18 folds and 24 folds of the number before culture; the NK activity (killing K562 cell) of malignant tumor patient's PBMC had grown up to 82% from 62% by CD 3AK cell transfusing, the even rising ratio is 32%.Conclusion (1) The UBMC is a potential and better source of predecessor for LAK and CD 3AK. (2) The NK activity of LAK and CD 3AK cells from UBMC reached their peaks at 11th day after culture, and the NK activity of CD 3AK cell is much greater than that of LAK cell. (3) The NK activity of malignant tumor patient's PBMC can be obviously elevated by transfusing CD 3AK cell. (4) The test of NK activity of PBMC of malignant tumor patient may become a recent objective immune index for tumor bio therapy.