The effects of two plant growth regulators 2,4-D and elongated fruit king on 5 different skin color of Cucurbita Pepo L were studied.The results show that 2,4-D and elongated fruit king could promote the growth of Cucurbita Pepo L and the effects of 2,4-D was significantly higher than that of elongated fruit king.The effects of different density of the elongated fruit king and 2,4-D on Cucurbita Pepo L were varied widely and the reaction of the same Cucurbita Pepo L to different plant growth regulators were diversed.The concentration of 2,4-D 5mg/kg was the best for Hanli and Heyuxiu and 10mg/kg was the best for Heyuqing and Heyuli.The concentration of elongated fruit king 25mg/kg was the best for Chunhu1.The above results would be helpful to study the effects of different plant growth regulators on different types of Cucurbita Pepo L under different environmental conditions.