Using a cluster random sampling method,208 college students were investigated by GRAT、GQ-6 and General Well-being Scale.Results:(1) There was no difference of GWB on genders,but female' s Gratitude was significantly higher than male's.(2) Gratitude was positively correlated with GWB,Gratitude was positively correlated with satisfaction and interest in life,depression or happy mood,energy,emotional and behavioral control,relaxation and tension(3) Satisfaction and interest in life,depression or happy mood two factors are the main factors affecting the level of gratitude.GWB was a significant positive predicator of Gratitude.Gratitude is an effective means to enhance the sense of subjective well-being.