Surface modified multi-functional PCL/TCP fibrous scaffolds

Event Abstract Back to Event Surface modified multi-functional PCL/TCP fibrous scaffolds Elbay Malikmammadov1, Tugba T. Endogan2, 3, Aysel Kiziltay2, 3, Vasif Hasirci1, 3, 4 and Nesrin Hasirci1, 3, 5 1 Middle East Technical University, Micro and Nanotechnology, Türkiye 2 Middle East Technical University, Central Laboratory, Türkiye 3 Middle East Technical University, BIOMATEN Center of Excellence in Biomaterials and Tissue Engineering, Türkiye 4 Middle East Technical University, Biological Sciences, Türkiye 5 Middle East Technical University, Chemistry, Türkiye Introduction: Porous and fibrous scaffolds prepared using various methods are essential components of tissue engineering. Poly(ε-caprolactone) (PCL), due to its biocompatibility and mechanical properties, is one of the most desirable polymers in scaffold preparation for bone tissue engineering applications, and there are many studies on modification of PCL to enhance its biocompatibility. In this study, porous and fibrous scaffolds of PCL containing TCP were prepared by wet spinning and gelatin was immobilized onto it to enhance cell attachment on the surface. In order to increase the local antimicrobial agent delivery to the application site, gelatin microvesicles carrying a model antibiotic were adhered on the scaffold surface. Scaffolds were incubated with human osteosarcoma cells Saos-2 to study its biocompatibility and the antimicrobial effect on E. coli. Materials and Methods: PCL scaffolds (PWS) and TCP-containing PCL scaffolds (PTWS) were prepared by wet spinning. Gelatin was immobilized on the scaffolds (PTWS-G) by grafting of acrylic acid onto it[1],[2]. Gelatin microvesicles which were prepared by water-in-oil emulsion (w/o) method were loaded with Ceftriaxone sodium and added on the samples via application of vacuum-pressure cycle to prepare the antimicrobial scaffolds (PTWS-G-M)[3]. The release kinetics of the drug from the scaffolds was studied. The effect of Ceftriaxone sodium on E.coli was determined by diffusion tests. Scaffolds were characterized by mechanical tests, scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS). Results and Discussion: SEM images showed that fibers of PWS and PTWS had defect-free, smooth surfaces with average diameters of approximately 200 µm (ImageJ, n=40). Compressive elastic moduli of PWS and PTWS were found to be 190 ± 50 kPa and 350 ± 10 kPa, respectively showing that incorporation of TCP improved the stiffness of PCL scaffolds significantly. EDX showed the presence of TCP on the fibers of PTWS. For gelatin immobilized samples, XPS results showed the presence of nitrogen of the immobilized protein on the surface of PTWS-G, confirming the presence of gelatin. C1s fitting was achieved to show amine bonding (Fig1). Scaffolds were seeded and incubated with Saos-2 cells. Cells tended to spread on the PCL, and the gelatin immobilization and TCP incorporation enhanced cell attachment and proliferation on the fibers (Fig 2). Conclusion: PCL scaffolds were prepared by wet spinning and modified with addition TCP, gelatin and antibiotic carrying microvesicles. TCP highly enhanced mechanical properties, gelatin improved cell adhesion and the antibiotic microvesicle system reduced the microbial load. These novel, multi-component and multi-functional scaffolds could be good candidates for bone tissue engineering applications. Authors are grateful for the Scientific and Technical Research Council of Turkey (TUBITAK) Grant (No 213M708) and METU Grant (BAP-07-02-2014-007-507).; We gratefully acknowledge the support to BIOMATEN through the Ministry of Development funds and by METU.; E.M. is grateful to TUBITAK for his scholarship.; Central Lab of METU for Characterization AnalysisReferences:[1] Krishnanand, K., Lal, D., Mishra, A., Gupta, B., Journal of Biomaterials and Tissue Engineering (2013) 3;2(7):233-239[2] Gupta, B., Krishnanand, K., Deapura, B. L, European Polymer Journal (2012) 48(11):1940-1948[3] Sezer, U A., Arslantunali, D., Aksoy E A., Hasirci V., Hasirci N, Journal of Applied Polymer Science (2014) 131;8, DOI: 10.1002/app.40110 Keywords: Tissue Engineering, in vitro, material design, Bioactive molecule Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: New Frontier Oral Topic: Surface and interfacial characterization Citation: Malikmammadov E, Endogan TT, Kiziltay A, Hasirci V and Hasirci N (2016). Surface modified multi-functional PCL/TCP fibrous scaffolds. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01555 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Elbay Malikmammadov Tugba T Endogan Aysel Kiziltay Vasif Hasirci Nesrin Hasirci Google Elbay Malikmammadov Tugba T Endogan Aysel Kiziltay Vasif Hasirci Nesrin Hasirci Google Scholar Elbay Malikmammadov Tugba T Endogan Aysel Kiziltay Vasif Hasirci Nesrin Hasirci PubMed Elbay Malikmammadov Tugba T Endogan Aysel Kiziltay Vasif Hasirci Nesrin Hasirci Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.