Damage Effects of Aβ_(25-35) on Primary Rat Cortical Neurons

Objective To observe the damage effects of Aβ25-35 on primary rat cortical neurons.Methods Rat cortical neurons were cultured and divided into 5 groups:the normal group(control),the Glu group,and 3 Aβ25-35 groups at ascending dosage,in each of which there were 6 wells of neurons.Morphological alterations of neurons were observed under inverted microscope after treatment,the neuron viability was detected,while LDH release was measured using LDH activity Kit.Results In contrast to controls,neurons in Glu group suffered significant damage with obvious morphological alterations,neuron viability rate was(53.1±5.5)%,LDH release increased and LDH viability rate was(39.9±2.9)U/g Prot with difference of statistical significance(P0.01).Neuron viability was found to have decreased in all Aβ25-35 groups,they were(69.3±5.9)%(P0.01),(52.7±3.0)%(P0.01),and(33.2±1.8)%(P0.01)respectively.There were no significant changes of LDH release found in both low and medium Aβ25-35 groups,except in the high Aβ25-35 group.LDH viability rates were(23.5±1.4)U/g Prot and(24.7±1.3)U/g Prot(P0.05)respectively.LDH activity in culture medium was(38.8±2.0)U/g Prot,which was higher than that in controls(P0.01),but equivalent to that in Glu group(P0.05).Conclusion Cultured rat cortical neurons can be impaired by Aβ25-35 in the dose relevant manner.Neuron viability decreasmay by about 50% after being treated with 1 μmol/L Aβ25-35 for 24 h,which can be applied as an Alzheimer's disease cell model in vitro.