Objective: To observe the level of IL 2 secreted by splenocytes of EL 4B7 1 + mixed culture immunized mice. Methods: CTLL 2 cells were used to detect the productivity of IL 2 secreted from splenocytes of EL 4B7 1 + cells mixed culture immunized mice. Results: The level of the production of IL 2 by splenocytes of EL 4B7 1 + mixed culture immunized mice was higher that of IL 2 produced by splenocytes of EL 4 immunized mice (P0 01) Conclusion: A high level of IL 2 can be secreted by splenocytes of EL 4B7 1 + cells mixed culture immunized mice.