间充质干细胞
骨髓
间质细胞
单元格排序
细胞
淋巴结间质细胞
细胞生物学
病理
免疫学
癌症研究
生物
医学
遗传学
作者
Hélène F.E. Gleitz,Inge Snoeren,Stijn N.R. Fuchs,Nils B. Leimkühler,Rebekka K. Schneider
出处
期刊:STAR protocols
[Elsevier]
日期:2021-06-01
卷期号:2 (2): 100538-100538
被引量:3
标识
DOI:10.1016/j.xpro.2021.100538
摘要
Bone marrow (BM) mesenchymal stromal cells play an important role in regulating stem cell quiescence and homeostasis; they are also key contributors to various hematological malignancies. However, human bone marrow stromal cells are difficult to isolate and prone to damage during isolation. This protocol describes a combination of mechanical and enzymatic isolation of BM stromal cells from human BM biopsies, followed by FACS sorting to separate stromal sub-populations including mesenchymal stromal cells, fibroblasts, and Schwann cells for single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Leimkühler et al. (2020).
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