川地163
胆道闭锁
医学
川地68
纤维化
IRF5公司
病理
巨噬细胞
M2巨噬细胞
免疫组织化学
染色
胃肠病学
内科学
免疫学
免疫系统
生物
先天免疫系统
干扰素调节因子
肝移植
体外
生物化学
移植
作者
Yifan Yang,Rui Dong,Chengchao Zheng,Shan Zheng,Gong Chen
标识
DOI:10.1016/j.jpedsurg.2017.08.045
摘要
Background/purpose Macrophages exert critical functions in liver homeostasis and have been proposed as potential targets in combatting fibrosis. We aimed to evaluate polarized functional status of liver infiltrated macrophages in infants with biliary atresia (BA). Methods Immunohistochemical staining for CD68, CD163, and IRF5 was performed in 40 BA infants. Liver biopsies were scored for fibrosis and tested for association with clinical biochemical characteristics. Results Developing lesions in BA liver progressively accumulated both CD163+ macrophages (M2) and IRF5+ macrophages (M1), while CD163 and IRF5 staining was stronger than the control group (p < 0.001). In BA, the higher staining density of CD163 and CD68 was related with elevated serum conjugated bilirubin level (p = 0.014 and 0.021, respectively). The CD163/IRF5 macrophages ratio was related with liver fibrosis scores (high vs. low, p = 0.004). Conclusions We demonstrated a strong presence of polarized macrophages in BA liver, and macrophage phenotypes were involved in disease development. The balance of different polarized macrophage subpopulations may play a key role in fibrogenesis of BA.
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