Effect of stress on the hatchability rates of stressed female broodstock and fry survival rates was investigated in this study. Breeding performance of Clarias gariepinus (0.95±0.32g) under different stress conditions; Starvation (ST) for 7 days, Unguided Daily sampling (UD) of fish on the last 7 days before hormonal injection for breeding , Low water level (LW) in fish tank for the last 7 days prior to breeding exercise and Control (CT). Experimental fish were injected hormone (Ovaprim) intramuscularly at an angle of 30 45 o of the dorsal fin to artificially induce them for spawning at the rate of 0.5ml/kg of fish. Weight of eggs stripped ranged between 70g and 90g with recorded mortalities except in CT. Hatchability rate varied significantly (p ST>UD. The significant better performance of fish under control experiment vindicated that stress is a major factor in broodstock performance which is capable of adversely affecting fry survival rates. It is therefore recommended that stress in fish should be minimized towards ensuring successful hatchery operations.