表观遗传学
DNA甲基化
生物
转录组
计算生物学
遗传学
胚胎干细胞
甲基化
DNA测序
基因
单细胞测序
抄写(语言学)
转录因子
基因组
表型
基因表达
外显子组测序
哲学
语言学
作者
Christof Angermueller,Stephen J. Clark,Heather Lee,Iain C. Macaulay,Mabel Teng,Xiaoming Hu,Felix Krueger,Sébastien A. Smallwood,Chris P. Ponting,Thierry Voet,Gavin Kelsey,Wolf Reik
出处
期刊:Nature Methods
[Springer Nature]
日期:2016-01-11
卷期号:13 (3): 229-232
被引量:596
摘要
We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
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