Determination of polysaccharide in fruit of Sorbus tianschanica Rupr. by phenol-sulfuric acid colorimetry

Objective To establish a quantitative analysis method for the determination of polysaccharide in fruit of Sorbus tianschanica Rupr..Methods Phenol-sulfuric acid method was used for the content determination of polysaccharide with glucose as reference substance.The detection wavelength was 490 nm.Results The polysaccharide content was 4.11%,the average recovery was 98.7%,RSD was 1.8%(n=6).Conclusion The method is rapid,accuracy and repeatable for determination of polysaccharide in fruit of Sorbus tianschanica Rupr..