The research on marigold extraction and saponification process about lutein

With the method of organic solvent extraction and mehtanol solution of potassium hydroxide saponification,this research studied the extraction and saponification process conditions of lutein in marigold flower.Research examined the effects of extraction agents types,extraction temperature,extraction time,and ratio of material to liquid on extraction,as well as the effects of saponification solution types,saponification solution concentration,saponification liquid dosage,saponification temperature and saponification time on saponification.On the base of single-factor test,the optimized conditions of extraction and saponification were:extraction agents was ethanol:petroleum ether(boiling range of 60 ℃90 ℃)(2 ∶1),expected liquid ratio was 1 ∶100,extraction temperature was 45℃,extraction time was 80min,saponification solution concentration was 20%,the amount of saponification solution was 0.2mL,saponification temperature was 40 ℃,saponification time was 80min.The use of ethanol-petroleum ether mixed solvent as extraction agent has the advantages of cheap,low toxicity,low boiling point,low solvent recovery costs,safe production.This extractant agent to extract lutein in industrial production was feasible.