Preparation and Chemical Compositions of Three Polysaccarides of Different Molecular Weights from Poria Cocos Wolf

Objective:Preparation and chemical compositions of three polysaccarides of different molecular weights and chemical compositions from Poria cocos Wolf were made.Methods:In this study,three polysaccharides were extracted from Poria cocos Wolf with water and sedimentation with alcohol of different concentrations.The polysacchardes were purified by sephadexG-75.Their chemical compositions were detected by TLC and GC-MS.Results:TLC and GC-MS revealed polysaccharide A was composed of mannose,glucose,galactose and fucose,and the mole ratio was 1.4∶ 1.17∶ 1∶ 4.21.Polysaccharide B was composed of mannose,glucose,galactose and fucose,and the mole ratio as 1∶ 2.36∶ 5.49∶ 2.34.Polysaccharide C was composed of mannose,glucose,galactose and fucose,and the mole ratio was 6∶ 81∶ 25∶ 1.Conclusion:The three polysaccharides were all composed of mannose,glucose,galactose and fucose with different mole ratios.