Expression of Lactoferrin mRNA in Mice Mammary Gland at Different Lactation Stages

To clarify the immune role of lactoferrin in mice mammary gland,half quantity RT-PCR was used to study the expression of lactoferrin mRNA in the mammary gland at different lactation stages of Kunming mice by taking the housekeeper gene GAPDH as a reference.The results showed that lactoferrin mRNA was expressed in mice mammary gland at different lactation stages.Lactoferrin mRNA expressive level decreased as the lactation progressed,its expressive level at early stage of lactation was significantly different from that at middle and later stage of lactation.The results suggested that lactoferrin was involved in the regulation of mammary immunity during the lactation of the tested mice.