The Electrofusion of Protoplasts of Banana

In order to optimize the parameters of banana's electrofusion,the protoplasts that isolated from Musa AAB Silk cv.Guoshanxiang and Musa itinerans Cheesm.AB Group were taken as material of electrofusion.It was shown that when electrofusion parameters were alternate current(AC) 200 V/cm,AC duration 30 s,direct current(DC) 1 500 V/cm,DC pulse with 40 μs and 2 times,the fusion efficiency was the best,which electrofusion frequency reached over 32.4% and vitality of protoplasts reached 81.4%.