The effect of Ara-C at low concentrations on CD147 expression in Jurkat cells

Objective:To investigate the effect of Ara-C at low concentrations on extracellular matrix metalloproteinase inducer(CD147) expression in Jurkat cells.Methods:Jurkat cells were divided into six groups:control group and cells treated with different concentrations of Ara-C at 1,5,10,20,and 30 ng/ml.CD147 mRNA and protein expression were assayed by RT-PCR,Western blot and flow cytometry,respectively.The colony formation in Jurkat cells which was stimulated with Ara-C or not was observed by inverted microscope.Results:Different concentrations of Ara-C significantly up-regulated the expressions of CD147 mRNA and protein,and increased the colony formation in Jurkat cells(P0.01).Conclusions:Ara-C at low concentrations can enhance the expression of CD147 and increase the adhesive force in Jurkat cells.