Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Sanguo Decoction by HPLC

Objective:To develop RP-HPLC for the determination of antioxidant contents including gallic acid and ellagic acid in Sanguo Decoction.Methods:The separation was performed on a welchrom C18(50mm×4.60mm,5μm)column with gradient elution.The mobile phase was acetonitrile-0.25% methanoic acid solution,and the flow rate was 0.85mL/min.The detection wavelength was set at 260nm.Billand column temperature was set at 25℃.Results:The linear ranges of gallic acid and ellagic acid were within 0.0500~0.7000mg and 0.0502~0.7140mg,respectively.The average recoveries for gallic and ellagic acid were 103.22%,97.49%(n=6).Conclusion:This method is suitable for the determinination of gallic acid and ellagic acid in Sanguo Decoction.