下调和上调
斑马鱼
三氯生
毒性
免疫系统
甲基化
核糖核酸
细胞生物学
发育毒性
化学
生物
医学
内科学
免疫学
基因
遗传学
生物化学
怀孕
胎儿
病理
作者
Qiuhui Qian,Qian Pu,Xin Li,Xingcheng Liu,Anyu Ni,Xiaofei Han,Zejun Wang,Xuedong Wang,Jin Yan,Huili Wang
出处
期刊:Chemosphere
[Elsevier]
日期:2024-02-01
卷期号:: 141395-141395
标识
DOI:10.1016/j.chemosphere.2024.141395
摘要
Triclosan (TCS), a prevalent contaminant in aquatic ecosystems, has been identified as a potential threat to both aquatic biota and human health. Despite its widespread presence, research into the immunotoxic effects of TCS on aquatic organisms is limited, and the underlying mechanisms driving these effects remain largely unexplored. Herein, we investigated the developmental and immune toxicities of environmentally relevant concentrations of TCS in zebrafish, characterized by morphological anomalies, histopathological impairments, and fluctuations in cytological differentiation and biomarkers following both acute (from 6 to 72/120 hpf) and chronic exposure periods (from 30 to 100 dpf). Specifically, acute exposure to TCS resulted in a significant increase in innate immune cells, contrasted by a marked decrease in T cells. Furthermore, we observed that TCS exposure elicited oxidative stress and a reduction in global m6A levels, alongside abnormal expressions within the m6A modification enzyme system in zebrafish larvae. Molecular docking studies suggested that mettl3 might be a target molecule for TCS interaction. Intriguingly, the knock-down of mettl3 mirrored the effects of TCS exposure, adversely impacting the growth and development of zebrafish, as well as the differentiation of innate immune cells. These results provide insights into the molecular basis of TCS-induced immunotoxicity through m6A-RNA epigenetic modification and aid in assessing its ecological risks, informing strategies for disease prevention linked to environmental contaminants.
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