Development of a time-resolved fluorescence microsphere immunochromatographic assay for simultaneous quantitative detection of urinary estrone-3-glucuronide and pregnanediol-3-glucuronide

We developed a rapid multiplex time-resolved fluorescent immunochromatographic assay for simultaneous quantification of urinary E1-3-G and PdG with a broad dynamic range, enabling non-invasive reproductive health monitoring.