印度刺猬
医学
骨关节炎
软骨内骨化
软骨细胞
软骨
刺猬信号通路
信号转导
内科学
细胞生物学
内分泌学
癌症研究
病理
解剖
生物
替代医学
作者
Lei Huang,Meng Jin,Ruiying Gu,Kunlin Xiao,Mingyu Lu,Xinyu Huo,Mengyao Sun,Zhi Yang,Zhiyuan Wang,Weijie Zhang,Liqiang Zhi,Ziang Meng,Jie Ma,Jianbing Ma,Rui Zhang
摘要
Osteoarthritis (OA), the most common type of arthritis, is an age-associated disease, characterized by the progressive degradation of articular cartilage, synovial inflammation, and degeneration of subchondral bone. Chondrocyte proliferation is regulated by the Indian hedgehog (IHH in humans, Ihh in animals) signaling molecule, which regulates hypertrophy and endochondral ossification in the development of the skeletal system. microRNAs (miRNAs, miRs) are a family of about 22-nucleotide endogenous non-coding RNAs, which negatively regulate gene expression. In this study, the expression level of IHH was upregulated in the damaged articular cartilage tissues among OA patients and OA cell cultures, while that of miR-199a-5p was the opposite. Further investigations demonstrated that miR-199a-5p could directly regulate IHH expression and reduce chondrocyte hypertrophy and matrix degradation via the IHH signal pathway in the primary human chondrocytes. The intra-articular injection of synthetic miR-199a-5p agomir attenuated OA symptoms in rats, including the alleviation of articular cartilage destruction, subchondral bone degradation, and synovial inflammation. The miR-199a-5p agomir could also inhibit the Ihh signaling pathway in vivo. This study might help in understanding the role of miR-199a-5p in the pathophysiology and molecular mechanisms of OA and indicate a potential novel therapeutic strategy for OA patients.
科研通智能强力驱动
Strongly Powered by AbleSci AI