硫化地杆菌
生物膜
菌毛
木桩
地杆菌
伞
粘附
微生物学
化学
细胞粘附
细菌
突变体
细胞外
细胞生物学
生物物理学
生物
生物化学
大肠杆菌
基因
遗传学
有机化学
作者
Xiaochun Chen,Guiqin Yang,Xiaoyun Quan,Shujin Zhu,Baoli Qin,Danyang Shou,Zhuang Li
标识
DOI:10.1016/j.scitotenv.2024.172242
摘要
Bacterial adhesion plays a vital role in forming and shaping the structure of electroactive biofilms that are essential for the performance of bioelectrochemical systems (BESs). Type IV pili are known to mediate cell adhesion in many Gram-negative bacteria, but the mechanism of pili-mediated cell adhesion of Geobacter species on anode surface remains unclear. Herein, a minor pilin PilV2 was found to be essential for cell adhesion ability of Geobacter sulfurreducens since the lack of pilV2 gene depressed the cell adhesion capability by 81.2 % in microplate and the anodic biofilm density by 23.1 % at −0.1 V and 37.7 % at −0.3 V in BESs. The less cohesiveness of mutant biofilms increased the charge transfer resistance and biofilm resistance, which correspondingly lowered current generation of the pilV2-deficient strain by up to 63.2 % compared with that of the wild-type strain in BESs. The deletion of pilV2 posed an insignificant effect on the production of extracellular polysaccharides, pili, extracellular cytochromes and electron shuttles that are involved in biofilm formation or extracellular electron transfer (EET) process. This study demonstrated the significance of pilV2 gene in cell adhesion and biofilm formation of G. sulfurreducens, as well as the importance of pili-mediated adhesion for EET of electroactive biofilm.
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