Determination of Monosaccharide and Disaccharide in Panax notoginseng by HPLC with SPE and ELSD

Objective An HPLC-ELSD method was established for the simultaneous quantitative analysis of glucose,fructose,sucrose from Panax notoginseng.Methods The chromatographic separation was achieved on an amino column using a mobile phase composed of a mixture of acetonitrile and water(75:25) at a flow rate of 0.8 mL/min by gradient elution.The ELSD drift tube temperature was set at 75 ℃ and the carrying gas was air.Results Under the above conditions,monosaccharides and disaccharides from Panax notoginseng could be well separated,and the spiked recoveries were 98.23%~101.46% with a RSD less than 3%(n = 3).Conclusion This method was accurate,rapid,simple and reproducible,and thus it would be suitable for the determination of monosaccharides,disaccharides from Panax notoginseng.