On the basis of lentinus edodes liquid fermentation, firstly domestication of selenium on lentinus edodes, then we performed deep fermentation technics experiments for lentinus edodes liquid of selenium-enriched, we studyed the tolerance and biotransformation of lentinus edodes mycelium to organic selenium in the process of fermentation. The results showed that lentinus edodes treated with selenium growed well in the solid mediums containing 60 mg/kg Na2SeO3, liquid seed mediums containing 80 mg/kg Na2SeO3 and liquid fermentation mediums containing 130 mg/kg Na2SeO3, and enrich selenium in the mycelium.