Multi-nucleosides in flesh of 18 kinds of longan(Dimocarpus longan Lour.) were well separated and measured quantitatively by HPLC.Results showed that nine nucleosides(uracil,cytidine,uridine,thymine,inosine,guanosine,thymidine,adenine,adenosine) were ascertained in Longan flesh.The contents of guanosine,uridine and adenosine were high with 30~70 μg/g fresh weight.The contents of thymine and cytidine took second place generally with 10~30 μg/g fresh weight.The contents of thymidine and uracil followed with 3~10 μg/g fresh weight.The contents of adenine and inosine were low.Assessed by contents of guanosine,uridine and adenosine,Shixia,Shuangmamu,Gushanerhao,Chuliang,Dawuyuan,Dongbi and Fuyan were good Longan varieties,which were mainly cultivated in Guangdong and Fujian province.