管家基因
生物
参考基因
基因
实时聚合酶链反应
基因表达
骨骼肌
遗传学
规范化(社会学)
计算生物学
分子生物学
解剖
人类学
社会学
作者
Yueying Wang,Yuqiang Zhao,Li Li Ji,Huijing Liu,Catherine W. Ernst,Xiaoran Liu,Guorong Liu,Yu X,Minggang Lei
出处
期刊:Gene
[Elsevier]
日期:2015-07-01
卷期号:565 (2): 235-241
被引量:21
标识
DOI:10.1016/j.gene.2015.04.016
摘要
Quantitative PCR (qPCR) requires a constantly expressed housekeeping gene as an internal control, the expression of which is similar in different biological samples. In the present study, we evaluated the applicability and compared the consistency of the gene expression of 16 reference genes, i.e., 10 common and 6 candidate genes, through qPCR assays in pig skeletal muscles at multiple developmental stages. The stability of these 16 potential reference genes was investigated using the geNorm and NormFinder methods. Our results indicated that DRAP1 and RNF7 were the most appropriate combination to normalize gene expression in the Yorkshire samples, the combination of DRAP1 and WSB2 were appropriate in the Tongcheng samples, H3F3A and DRAP1 in prenatal periods, DRAP1 and RNF7 in postnatal periods, and the combination of DRAP1 and WSB2 was most suitable for accurate normalization in whole samples. This result provides a strong basis for similar studies in pigs that explore the molecular mechanisms underlying skeletal muscle development.
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