核酸
寡核苷酸
血脑屏障
细胞凋亡
DNA
材料科学
纳米技术
癌症研究
生物物理学
化学
细胞生物学
医学
生物化学
生物
中枢神经系统
内科学
作者
Wenyan Yu,Cuiping Xuan,Bingbing Liu,Lei Zhou,Na Yin,Enpeng Gong,Zhenzhong Zhang,Yinchao Li,Kaixiang Zhang,Jinjin Shi
出处
期刊:Nano Research
[Springer Nature]
日期:2022-05-24
卷期号:16 (1): 735-745
被引量:5
标识
DOI:10.1007/s12274-022-4402-7
摘要
Antisense oligonucleotide (ASO) for anti-apoptosis is emerging as a highly promising therapeutic agents for ischemic stroke with complex pathological environment. However, its therapeutic efficacy is seriously limited by a number of challenges including inefficient internalization, low blood-brain barrier (BBB) penetration, poor stability, and potential toxicity of the carrier. Herein, a carrier-free programmed spherical nucleic acid nanostructure is developed for effective ischemic stroke therapy via integrating multifunctional modules into one DNA structure. By co-encoding caspase-3-ASO and transferrin receptor (TfR) aptamer into circle template, the spherical nucleic acid nanostructure (TD) was obtained via self-assembly. The experimental results demonstrated that the developed TD displayed efficient BBB penetration capability (6.4 times) and satisfactory caspase-3 silence effect (2.3 times) due to the dense DNA packaging in TD. Taken together, our study demonstrated that the carrier-free programmed spherical nucleic acid nanostructure could significantly improve the therapeutic efficacy of ischemic stroke and was a promising therapeutic tool for various brain damage-related diseases.
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