Currently, high strength steel is not used for steel bridges in Korea, except for the SM570 high strength steel in very isolated cases. The study aimed to promote the active adaptation of high strength steel for long-span steel bridges. Thus, the fatigue behavior of SM570 and POSTEN80 high strength steel was investigated. For the experimental study, the butt welded joints samples were manufactured. Likewise, regular amplitude tensile fatigue tests were conducted. Test results, e.g., location of fatigue cracks and their propagation were compared with the findings of other researchers. After analyzing the effects of fatigue strength, e.g., static tensile strength and plate thickness of base metal, basic data for fatigue design criteria of SM570 and POSTEN80 high strength steel were presented.