生物化学
新陈代谢
微生物群
牛磺酸
肠道微生物群
酶
水解
化学
盐(化学)
胆汁酸
生物
肠道菌群
氨基酸
生物信息学
物理化学
作者
Kristoffer R. Brandvold,Carson J. Miller,Regan F. Volk,Bryan Killinger,Christopher Whidbey,Aaron Wright
出处
期刊:ChemBioChem
[Wiley]
日期:2021-02-10
卷期号:22 (8): 1448-1455
被引量:10
标识
DOI:10.1002/cbic.202000748
摘要
Abstract Microbial bile salt hydrolases (BSHs) found in the intestine catalyze the deconjugation of taurine‐ and glycine‐linked bile salts produced in the liver. The resulting bile salts are biological detergents and are critical in aiding lipophilic nutrient digestion. Therefore, the activity of BSHs in the gut microbiome is directly linked to human metabolism and overall health. Bile salt metabolism has also been associated with disease phenotypes such as liver and colorectal cancer. In order to reshape the gut microbiome to optimize bile salt metabolism, tools to characterize and quantify these processes must exist to enable a much‐improved understanding of how metabolism goes awry in the face of disease, and how it can be improved through an altered lifestyle and environment. Furthermore, it is necessary to attribute metabolic activity to specific members and BSHs within the microbiome. To this end, we have developed activity‐based probes with two different reactive groups to target bile salt hydrolases. These probes bind similarly to the authentic bile salt substrates, and we demonstrate enzyme labeling of active bile salt hydrolases by using purified protein, cell lysates, and in human stool.
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